estrogen receptor expression in breast cancer cell lines
In breast cancer cells, 17-beta-estradiol (E2) upregulates the expression of insulin receptor substrate 1 (IRS-1), a molecule transmitting insulin-like growth factor-I (IGF-I) signals through the PI-3K/Akt survival pathways. The stimulation of IRS-1 by E2 has been documented on the transcriptional level. 2004. Estrogen receptor inhibits 17-estradiol-stimulated proliferation of the breast cancer cell line T47D.Estrogen induces c-myc gene expression via an upstream enhancer activated by the estrogen receptor and the AP-1 transcription factor. Estrogen receptor/progesterone receptor (ER/PR) expressing breast cancers are treated with endocrine therapy.1991]). Additionally, reports that FRA expression levels may be associated with disease stage or survival in ovarian cancer or non-small cell lung cancer, suggest that FRA may be a High N-Acetyltransferase 1 5-androstane-3, Expression is Associated with Estrogen Receptor Expression in Breast Tumors, but is not Under Direct Regulation by Estradiol, 17-Diol, or Dihydrotestosterone in Breast Cancer Cells. Abstract: Although expression of estrogen receptor (ER), progesterone receptor (PR), and cell proliferation marker Ki67 serve as predictive and prognostic factors in breast cancers, little is known about their roles in normal breast tissue. ER-negative primary breast cancers and cell lines showed increased Src levels and/or activity compared with ER-positive cancers and cells.2000. Quantitative analysis of estrogen receptor-alpha and -beta messenger RNA expression in breast carcinoma by real-time polymerase Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. Latest Estrogen Receptor Positive Breast Cancer Information - Duration: 25:15.Detecting and measuring estrogen receptor expression in parathyroid tumors.wmv - Duration: 27:54. Visiopharm 809 views. Data from gene expression microarrays were used to compare the global structures of the transcriptomes of three estrogen receptor alpha positive (ER) human breast cancer cell lines (MCF-7, T47D, ZR-75-1) and 13 human breast tumors (11 ER 2 ER BACKGROUND OBJECTIVE: Gene transfection is a major approach in studies of estrogen receptor (ER)expression in breast cancer cells,and ERs functional mechanism. This study was designed to detect the transfection efficiency of different human breast cancer cell lines mediated by FuGENE6 The growth of estrogen receptor (ER)-positive breast cancer cells is hormonally regulated, but the majority of breast cancers are ER negativeFurther, a panel of TNBC cell lines exhibit significant levels of ER protein. To assess ER effects on proliferation, ER expression in TNBC cells was -79 Manuscript 3 Title: Attenuation of Estrogen Receptor (ER) Signaling by Selenium in Breast Cancer Cells via Downregulation of ER Gene ExpressionThe related protein, cytokeratin 19 (Ck 19), is regulated by estrogen in breast cancer cell lines (Choi et al 2000). Here, for the first time, we report about cNOS expression in 10 of 16 human breast cancer cell lines, cNOS expression correlates strongly with expression of estrogen receptor (ER).
Competitive reverse transcription - polymerase chain reaction (cRT-PCR) The expression of the positive control genes GREB1 and TFF1, and target genes BMP4 and BMP7 was assessed with qRT-PCR from six breast cancer cell lines: estrogen receptor (ER) positive BT-474, MCF-7, MDA-MB-361, T-47D and ZR-75-30, and ER negative MDA-MB-231. Download Sport Book gene expression in estrogen receptor positive and negative human breast cancer cell lines Free.Interest in the cell cycle has grown explosively in recent years as a result of the identification of key cell cycle regulators and their substrates. Sulforaphane and Epigallocatechin Gallate Restore Estrogen Receptor Expression by Modulating Epigenetic Events in the Breast Cancer Cell Line MDA-MB-231: A Systematic Review and Meta-Analysis. Cells of breast, endometrium, ovary and prostate, were grown in the laboratory. Estrogen (estradiol) was added to the cells.inhibits the proliferation of breast cancer cells, a variety of cancer cell lines with different receptors and different expression of genes were exposed to progesterone. AF-sensitive breast cancer cells examined to this point express estrogen receptor (ER).
SULT1A1: sulfotransferase 1A1 DSB: double strand breaks .A. Monks, E. Harris, C. Hose et al Genotoxic profiling of MCF-7 breast cancer cell line elucidates gene expression modifications underlying Highlights Np63 induces quiescence in MCF7, luminal breast cancer cells but not in others. Stemness is not affected by Np63 in two mutant p53-expressing breast cancer lines.Inducible expression of Np63 in MCF7 estrogen receptor positive (ER) luminal breast Immunohistochemistry of estrogen (ER) and progesterone (PR) receptors in breast cancer is an established method of predicting responsiveness to hormonalThe state of PR-B dominance, like in the cell line HEC-1A, is less invasive than cell lines with predominantly PR-A expression . Most of these cases are ER-positive, meaning that there are estrogen receptors on the surface of the cell that bind to estrogen. If your doctor suspects breast cancer, you will likely have a biopsy. A doctor will test your cells to see if they are cancerous. The resulting Nf1 indels induced highly penetrant, aggressive mammary adenocarcinomas that express estrogen receptor and progesterone receptor. We identified distinct Nf1 isoforms that were altered during tumorigenesis. To evaluate NF1 in human breast cancer Whilst most of the mechanistic data have been obtained using breast cancer cell linesAndrogen receptor expression varies according to breast cancer subtype Androgen receptor (AR) is expressed most commonly in estrogen receptor (ER) tumors, in approximately half of HER2 and a. It has been shown that estrogen is related to breast cancer development through binding to its receptors. In order to uncover the estrogen effects on adenosine receptor expression, estrogen-positive MCF-7 cells were used to treat with agonist and antagonist of estrogen and thenthat ER positive (ER) breast cancers proliferate more slowly than ER- breast cancers.For the T47D and ZR-75 cell lines, loss of ER was associated with a decrease in doubling timeJordan, VC, Welshons, WV, Curran, EM, Ellis, MJ El-Ashry, D 2004, Estrogen receptor expression and Loss of estrogen receptor (ER) expression and gain of TWIST (TWIST1) expression in breast tumors correlate with increased disease recurrence andIn agreement with these molecular events, TWIST expression was inversely correlated with ER expression in both breast cancer cell lines We show here that the absence of estrogen receptor expression in human breast cancer cell lines is associated with higher levels of functional EGF receptor protein and mRNA. ER as well as progesterone receptor (PgR) expression are useful in predicting response to endocrine therapy. In this study a long-term estrogen deprivation (LTED) model was used to study the three commonly used breast cancer cell lines MCF-7, BT-474 and MDA-MB-231. Estrogen receptor beta as a novel target of androgen receptor action in breast cancer cell lines. Pietro Rizza, Ines Barone, 9 authors SebastianoAndrogens down-regulate bcl-2 protooncogene expression in ZR-75-1 human breast cancer cells. J Lapointe, A Fournier, V Richard, C Labrie. In human breast cancer cell lines, RhoB attenuation was associated with reduced expression of both ER and PR, whereas elevation of RhoB wasVol 14: Calcitriol restores antiestrogen responsiveness in estrogen receptor negative breast cancer cells: A potential new therapeutic approach. Human breast cancer cell (BCC) lines are used extensively in biomedical research and are classified as estrogen receptor (ER)-positive orused flow cytometry (FCM), reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting (WB) to assess ER expression in human BCC lines The expression and stability of the estrogen receptor (ER) is the result of a complex process that is modulated by estrogens and antiestrogens.The models presented here describe the response of two human breast cancer cell lines in short-term studies.on the expression of the high-affinity, metastasis-associated laminin receptor in two human breast carcinoma cell linesThe T47D cell line contains estrogen and progesterone receptors, but therelate functionally to the difference in the clinical aggressiveness between classes of breast cancers. In addition, estrogen receptor (ER) is an important prognostic factor in breast cancer cells. Therefore it is important to determine the Bcl-2 and CCND1 expression in MCF7, T47D and MDA-MB-468 breast cancer cell lines with different ER status following Adriamycin (ADR) treatment. The relationship between estrogen receptor (ER) and MAPT in breast cancer is unclear.In our study in ER-positive and MAPT-positive breast cancer cell lines, expression of MAPT protein isoforms of less than 70 kDa, which have a large impact on sensitivity to taxanes, was affected by ER (3) was further supported by epidemiological data (4) and the therapeutical efficacy of ovariectomy and antiestrogen therapy (5). More direct evi-dence was obtained with estrogen receptor (ER)-positive breast cancer cell lines in which estrogens were found to stimulate the expression of specific genes Obesity is associated with a worse breast cancer prognosis, while greater breast tumor estrogen receptor beta (ER) expression isFour human mammary tumor cell lines representing the major breast cancer subtypes (SKBR3, MCF-7, ZR75, MDA-MB-231) and mammary tumor cells from Four human mammary tumor cell lines representing the major breast cancer subtypes (SKBR3, MCF-7, ZR75, MDA-MB-231) and mammary tumor cells from MMTV-neu miceExpression of estrogen receptor beta isoforms in normal breast epithelial cells and breast cancer: regulation by methylation. 2.4.1. Cell lines Six human lung, prostate and breast cancer cell lines A549 (CCL M.K. Verma, Y. Miki, K. Abe, S. Nagasaki, H. Niikawa, S. Suzuki, et al Co- expression of estrogen receptor beta and aromatase in Japanese lung cancer patients: gender-dependent clinical outcome Oncogene 19:315322. beta in the regulation of estrogen receptor expression in Moggs JG, Orphanides G. 2001. Estrogen receptors: the MCF-7 breast cancer cell line. Endocrinology Orchestrators of pleiotropic cellular responses. The presence of estrogen receptors (ERs) in breast carcinomas is important for clinical response to endocrine therapy.The endocrine therapy responsive breast cancer cell lines MCF-7 and T47-D were positive for both ER- and c-erbB-4 expression, while the endocrine therapy non-responsive In the present study, the expression of estrogen receptor (ER) and ER isoforms in ER-positive (MCF7, T-47D and ZR-75-1) and ER-negative (MDA-MB-231, SK-BR-3, MDA-MB-453 and HCC1954) breast cancer cell lines was investigated. On the other hand, Estrogen Receptor (ER), an important prognostic factor is differentially expressed in breast cancer cells.Interestingly, higher level of p53 protein expression was detected after ADR exposure in all three cell lines. In this study we provide the first gene array profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene expression.Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity.
Cancer Res. 200565:6557-6567. Uptake of estrogen precursors is important for cell proliferation in estrogen receptor (ER)-positive breast cancer.The expression of SLCO1A2, 2B1 and 3A1 mRNAs was detected in normal breast tissues, malignant breast tissues and MCF-7 cells (a breast cancer-derived cell line). Estrogen receptor beta as a novel target of androgen receptor action in breast cancer cell lines.ER-beta expression was examined in human breast cancer cell lines using real-time PCR, Western blotting and small interfering RNA (siRNA) assays. We have identified and characterized a unique subclone of the MCF-7 human breast cancer cell line, named MCF-7:2A, which growsThe MCF-7:2A cells express high levels of estrogen receptor (ER 477 fmol/mg protein), which can be reduced by growth in 10 nM 17-estradiol (201 fmol/mg protein). For example, ectopic expression of miRNA-24-2 star strand in the estrogen receptor (ER) positive breast cancer cell line MCF-7, results in suppression of cell survival, through the targeted suppression of protein kinase C (PKC), and decreased tumor formation when injected into nude mice . Profound differences in the expression of estrogen and progesterone receptors and estrogen metabolizing enzymes in breast cancer cell lines (38) indicate that choosing the proper cell model is of great importance. 6.8.2 How is ER and PR Expression by IHC Quantified? Most Pathology testing labs use IHC for Estrogen receptors (ER) and Progesterone receptors (PR).Immunohistochemistry (IHC) detects the HER2 protein that is present on the cell membrane of breast cancer cells.